Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. , Oxon, UK). The area under the curve (AUC) for the comparison of the growth rates was determined using. plates can be used in the SPECTRAmax PLUS 384. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). TGFα release was calculated as described in the Results. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. 2. mums. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Values were determined according to a standard curve (log/log curve-fit). The absorbances were being measured at 515 nm. Molecular Devices SPECTRAmax. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. SpectraMax Multi-Mode Microplate Readers. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 303 unable to cal dark current. Immunofluorescence and Aggregation Analysis. Features showcased by this model include. Lactate Dehydrogenase (LDH) Release. Subsequently, the mixture was heated at 100 °C for 10. 3. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. TDP-43 stress granular cells were treated with 70% ice-cold. Donor filter: 620 nm. 10. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). MMP‑1 inhibition assay. 3 mm. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 8. SpectraMax 190 reads 96-well and strip-well plates. Oxidative stress induction by H 2 O. The assays contained 0. . Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Molecular Devices SpectraMax Gemini XS Microplate Reader. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Emission. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. or microplate reader (e. 316 grating motor driver faulty. 5 g/L-EDTA 0. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Call 1-800-745-2710. TDP-43 stress granular cells were treated with 70% ice-cold. Molecular Devices Spectramax 340PC instructions. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Immunofluorescence and Aggregation Analysis. Quick View. Lactate Dehydrogenase (LDH) Release. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Description 1. Quick View. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Data were expressed as the mean percent viable cell vs. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). 340/70 nm. 5 mL of 1 M acetate buffer (pH 4. Alkaline phosphatase (ALP) activity. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Microscopy. SPECTRAMAX 340. ATI/240, 250. 314 low lamp level warning. The reaction mixture was incubated for 5 min. 190-1000 nm. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 190–850 nm <± 1. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Awareness Technology Inc. The plates were incubated for 4 additional hours at 37°C. application or budget. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Warranty. 2 0. 4. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 313 reference gain check fail. used in the SPECTRAmax PLUS. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. SPECTRAmax® 190. SpectraMAX 340 microtiter plate reader. MCE has not independently confirmed the accuracy of these methods. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. 2. Male 8 pin mini Din to Female 9D. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 0% and ± 0. using SpectraMax i3 (Molecular devices, San Jose, USA). 3: The cuvette chamber. Table 1. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Quick View. ). reader (Molecular Devi ces). Stat Fax 4700 Microstrip Reader. , Sunnyvale, CA). edu Contact Phone: 216-368-8962. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Stat Fax 4700 Microstrip Reader. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. SpectraMax® Microplate Readers and SoftMax® Pro Software. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Page: 1/6. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Details. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. chen@case. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. Call 1-800-745-2710. Manager: Yinghua Chen, PhD Contact Email: yinghua. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Acceptor: 665/10 nm. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Details. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. SpectraMax Multi-Mode Microplate Readers. A. Percent viability was calculated by comparison with the control group. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Each experiment was performed in duplicate. StakMax. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. P. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 2. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. This was followed by an additional incubation at 37 °C for 30–60 min. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Molecular Devices Corporation 1311. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. TDP-43 stress granular cells were treated with 70% ice-cold. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. Briefly, cells were seeded into a. Details. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Cet appareil ISM est confomre à la norme NMB-001 du Canada. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. more on but is otherwise untested and sold AS-IS. 1: SpectraMax Plus 384 components. MCE has not independently confirmed the accuracy of these methods. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 2. , Fremont, CA, USA). The reaction mixture was incubated for 5 min. 0200-6117. To. control. Homeostatic model assessment for insulin. , Sunnyvale, CA) at 490 nm. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The plate was covered and incubated for one hour at room temperature. 0 2. Samples were plated in duplicate (r=0. SpectraMax QuickDrop Micro-Volume Spectrophotometer. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Detection limits. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. One plate carrier adapter is provided with the instrument. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. using SpectraMax i3 (Molecular devices, San Jose, USA). An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. These isotypes cause antigen cross-linking by multimeric binding and self. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Call 1-800-745-2710. To measure the cell biomass (g/L),. Microplate Spectrophotometer Operator’s Manual. 340 nm / 70 nm. 5 stars - 1602 reviewsThis assay typically develops in 1. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Endpoint, kinetic, spectrum. 0. , Sunnyvale CA). Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Create custom fluorescence assays without having to purchase expensive filter sets. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The SPECTRAmax 340PC can measure. . SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. 5mM), and changes in OD 405 nm. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Analysis of ALA by Ehrlich’s Reagent Assay. Crowder, Ziqian Zeng, Alissa N. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Not. Wavelength range: 340-850 nm. Binding affinity for denosumab. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Read one sample or up to 384. Microplate Readers. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. 0200-7027. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Molecular Devices SpectraMax M5e Microplate Reader. Manager: Yinghua Chen, PhD Contact Email: yinghua. The adapter is. Our absorbance plate readers feature our PathCheck Sensor technology and. The values were corrected for nonspecific background by subtracting the OD 650. They are for reference only. , Sunnyvale, CA). 024). Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. 0 OD. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. This test was performed in duplicates. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. 7. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Analysis of ALA by Ehrlich’s Reagent Assay. SoftMax Pro. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Nitric Oxide Assay. Molecular Devices Corporation 1311. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Service parts for these models are not being produced and are no longer in stock. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 5 - 2. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. reader (SpectraMax 340; Molecular devices, LLc). LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. 5. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. #N207) LAL Reagent Water (Lonza cat. Software (SoftMax Pro ® software or comparable plate reader software). The ratio of red to green fluorescence intensity was calculated. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Data were expressed as the mean LPS-treated cells (100%) vs. A. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. 304 signal level saturation. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Stat Fax 3200 Microplate Reader. 2 Chemical Reagents 1. 4. used. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. Each experiment was performed in duplicate. Statistical analysis. Water – distilled water or equivalent. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. 1. Donor: 620 nm. Spectrophotometer (SpectraMax 340). #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. All strains were tested three times and the results averaged. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. Donor: 616/10 nm. One plate carrier adapter is provided with the instrument. 5. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. TDP-43 stress granular cells were treated with 70% ice-cold. Data System. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Cell culture 3T3 fibroblasts and RAW 264. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Microplate Readers. 30. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. The first time you replace an OEM lamp you must use the N-34-MLA. 2. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Streamline GLP/GMP compliance. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Acceptor filter: 665/10 nm. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. albicans pre. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Molecular Devices Spectramax 340PC instructions. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). 06 ng/mL; FSH: 0. 5. The sensitivities of the hormones are Prolactin: 0. The SPECTRAmax 340PC can measure. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. View the Molecular Devices website. 81. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Assay setup for a 384-well low-volume plate. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Or scan up to 384 wells at any time to determine the best assay conditions. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. SpectraMax ® Plus 384 optics. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. chen@case. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. \r plates can be used in the SPECTRAmax PLUS 384. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. 1% PEG 8000 at 37°C. 5 mL sample or standard solution was well mixed with 0. Anti-proteolytic activity. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium.